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Nagesh, M.
- Evaluation of Beneficial Fungi in Combination with Organics against Root-knot Nematode, Meloidogyne incognita, in Fcv Tobacco Nurseries
Abstract Views :214 |
PDF Views:134
Authors
S. Ramakrishnan
1,
M. Nagesh
2
Affiliations
1 CTRI Research Station, Hunsur, Karnataka, IN
2 National Bureau of Agriculturally Important Insects, Bangalore 560 024, Karnataka, IN
1 CTRI Research Station, Hunsur, Karnataka, IN
2 National Bureau of Agriculturally Important Insects, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 4 (2011), Pagination: 311-315Abstract
FCV tobacco is an important commercial crop grown in Karnataka under rain fed situations. Root-knot nematode is a limiting factor causing yield reduction in both nursery and main field crop to the tune of 59.4% and 52.9%, respectively. As an alternative to nematicides of chemical origin, beneficial fungi such as Paecilomyces lilacinus and Glomus fasciculatum were evaluated along with organic amendments viz., neem cake and vermicompost as individual application and also in rational combinations against Meloidogyne incognita in FCV tobacco nurseries. Results revealed that application of P. lilacinus strain NBAII PLFT5 @ 100g/m2 recorded 31.3% increase in healthy transplants compared to check. Combined application of P. lilacinus with neem cake @ 1kg/m2 recorded 34.4% increase in healthy transplants count and was on par with P. lilacinus with vermicompost @ 1kg/m2. Application of P. lilacinus + neem cake @ 1kg/m2 and P. lilacinus + vermicompost @ 1kg/m2 significantly reduced ischolar_main-knot index to 1.89 compared to 2.05 in carbofuran @ 50g/m2 treated beds (standard chemical check) and 3.86 in untreated check. Similarly, these two treatments were on par with each other in significantly reducing the number of egg masses/g ischolar_main and final soil nematode population.Keywords
Bio-management, Glomus fasciculatum, Paecilomyces lilacinus, Root-knot Nematode, FCV Tobacco, Neem Cake, Vermicompost.References
- Bagyaraj, D. J., Manjunath, A. and Reddy, D. D. R. 1979. Interaction of vesicular arbuscular mycorrhiza with ischolar_mainknot nematodes in tomato. Plant and Soil, 51: 397–403.
- Cooper, K. M. and Grandisons, G. S. 1986. Interaction of vesicular-arbuscular mycorrhizal fungi and ischolar_main knot nematode on cultivars of tomato and white clover susceptible to Meloidogyne hapla. Annals of Applied Biology, 108: 555–565.
- Ekanayake, H. M. R. K. and Jayasundara, N. J. 1994. Effect of Paecilomyces lilacinus and Beauveria bassiana in controlling Meloidogyne incognita on tomato in Sri Lanka. Nematologia mediteranea, 22: 87–88.
- Hussaini, S. S. 1983. Quantification of ischolar_main-knot nematode damage on FCV tobacco. Tobacco Research, 19: 61-65. Jonathan, E. I., Padmanabhan, D. and Ayyamperumal, A. 1995. Biological control of ischolar_main-knot nematode on betel vine, Piper betle by Paecilomyces lilacinus. Nematologia Mediterranea, 23: 191–193.
- Nagesh, M. and Reddy, P. P. 2004. Biochemical changes in Glomus fasciculatum colonized ischolar_mains of Lycopersicon esculentum in the presence of Meloidogyne incognita. Indian Journal of Experimental Biology, 42: 721-727.
- Nagesh, M., Reddy, P. P., Kumar, M. V. and Nagaraju, B. M. 1999. Studies on correlation between Glomus fasciculatum spore density, ischolar_main colonization and Meloidogyne incognita infection on Lycopersicon esculentum. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, 106: 82–87.
- Nagesh, M. and Singh, K. P. 2004. Bio-management of Meloidogyne incognita on Polianthes tuberosa using Glomus mosseae and Pochonia chlamydosporia as bulb dressing in combination with neem cake. Journal of Ornamental Horticulture, 7: 45-51.
- Ramakrishnan, S., Hussaini, S. S., Viswanath, S. M. and Shenoi, M. M. 1998. Effect of Basamid G for the control of ischolar_mainknot nematodes in FCV tobacco nursery, pp. 121–124. In: Dhawan, S. C. and Kaushal, K. K. (Eds): Proceedings of the National Symposium on rational approaches in nematode management for sustainable agriculture. Gujarat Agricultural University, Anand.
- Rao, M. S. and Gowen, S. R. 1998. Bio-management of Meloidogyne incognita on tomato by integrating Glomus deserticola and Pasteuria penetrans. Zeitschrift fur Pflanzenkrankheiten und Pflanzenschutz, 105: 49–52.
- Rao, M. S., Reddy, P. P. and Nagesh, M. 2000. Management of Meloidogyne incognita on tomato by integrating Glomus mosseae with Pasteuria penetrans under field conditions. Pest Management in Horticulural Ecosystems, 6: 130–134.
- Ray, S. and Dalei, B. K. 1998. Vam for ischolar_main knot-nematode management and increased productivity of grain legumes in Orissa. Indian Journal of Nematology, 28: 23–28.
- In silico Docking Studies on Cytochrome P450 Enzymes of Helicoverpa armigera (Hubner) and Trichogramma cacoeciae Marchal and Implications for Insecticide Detoxification
Abstract Views :436 |
PDF Views:150
Authors
K. P. Dhanya
1,
Madhusmita Panda
1,
S. K. Jalali
1,
N. K. Krishna Kumar
1,
R. Gandhi Gracy
1,
T. Venkatesan
1,
M. Nagesh
1
Affiliations
1 Molecular Entomology Laboratory, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
1 Molecular Entomology Laboratory, National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 27, No 1 (2013), Pagination: 1-9Abstract
In silico docking of cytochrome P450 monooxygenase (CYP450) of an insect, Helicoverpa armigera (Hübner) and a parasitoid, Trichogramma cacoeciae Marchal was studied with two insecticides, monocrotophos and fenvalerate. The CYP450 sequences of H. armigera (CYP9A12), T. cacoeciae (CYP4G12) and a human microsomal sequence CYP3A4, as positive control were retrieved from NCBI’s GenBank database. The structure, as predicted by SOPMA, of CYP450 in H. armigera contained 78.7% helix and 43.3% sheets, while that of T. cacoeciae contained 60.6% helix and 68.5% sheets. The three-dimensional molecular models of CYP450 of H. armigera and T. cacoeciae indicated that 96.5 and 97.2% residues, respectively, were in the most favored region. The docking studies revealed that the binding energy of H. armigera was -3.50 and -7.65 kcal/mole compared to the binding energy of T. cacoeciae -2.96 and -5.28 kcal/mole for monocrotophos and fenvalerate, respectively, inferring stronger interaction of H. armigera CYP450 with the insecticides and thereby higher potential for resistance in H. armigera.Keywords
Cytochrome P450, Helicoverpa armigera, Trichogramma cacoeciae, In silico Molecular Docking.References
- Armes NJ, Jadhav DR, DeSouza KR. 1996. A survey of insecticides resistance in Helicoverpa armigera in the Indian subcontinent. Bull Ent Res. 86: 499–514.
- Bachar O, Fischer D, Nussinov R, Wolfson HJ. 1993. A Computer vision based technique for 3-D sequence independent structural comparison of proteins. Protein Eng. 6: 279–288.
- Baudry J, Li W, Pan L, Berenbaum MR, Schuler MA. 2003. Molecular docking of substrates and inhibitors in the catalytic site of CYP6B1, an insect cytochrome P450 monooxygenase. Protein Eng. 16: 577–587.
- Baudry J, Rupasinghe S, Shuler MA. 2006. Classdependent sequence alignment strategy improves the structural and functional modeling of P450s. Protein Eng Des Sel. 19: 345–353.
- Bikadi Z, Hazai E. 2009. Application of the PM6 semiempirical method to modeling proteins enhances docking accuracy of AutoDock. J Cheminf. 1: 15.
- Bull DL, House VS. 1983. Effects of different insecticides on parasitism of host eggs by Trichogramma pretiosum Riley. Southwestern Entomol. 8: 46–53.
- Cariño FA, Koener JF, Plapp Jr. FW, Feyereisen R. 1994. Constitutive over expression of the cytochrome P450 gene CYP6A1 in a house fly strain with metabolic resistance to insecticides. Insect Biochem Mol Biol. 24: 411–418.
- Daborn PJ, Yen JL, Bogwitz MR, Le Goff G, Feil E, Jeffers S. 2002. A single p450 allele associated with insecticide resistance in Drosophila. Science 297: 2253–2256.
- Daborn PJ, Lumb C, Boey A, Wong W, ffrench-Constant RH, Batterham P. 2007. Evaluating the insecticide resistance potential of eight Drosophila melanogaster cytochrome P450 genes by transgenic overexpression. Insect Biochem Mol Biol. 37: 512–519.
- de Graaf C, Vermeulen NP, Feenstra KA. 2005. Cytochrome P450 insilico: an integrative modeling approach. J Med Chem. 48: 2725–2755.
- Edgar RC. 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 32, 1792–1797.
- Feyereisen R, Insect P450 enzymes, Ann Rev Entomol. 44: 507–733.
- Jalali SK, Singh SP, Venkatesan T, Murthy KS, Lalitha Y. 2006. Development of endosulfan tolerant strain of an egg parasitoid Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae). Indian J Exp Biol. 44: 584–590.
- Jones RT, Bakker SE, Stone D, Shuttleworth SN, Boundy S, McCart C, Daborn PJ, ffrench-Constant RH, van den Elsen JM. 2010. Homology modelling of Drosophila cytochrome P450 enzymes associated with insecticide resistance. Pest Mgmt Sci. 66: 1106–1115.
- Korytko PJ, Scott JG. 1998. CYP6D1 protects thoracic ganglia of houseflies from the neurotoxic insecticide cypermethrin. Arch Insect Biochem Physiol. 37: 57–63.
- Kranthi KR, Jadhav DR, Kranthi S, Wanjari RR, Ali SS, Russell DA. 2002. Insecticide resistance in five major insect pests of cotton in India. Crop Prot. 21: 449-460.
- Laskowski RA, MacArthur MW, Moss DS, Thornton JM. 1993. PROCHECK: a program to check the stereochemical quality of protein structures. J Appl Cryst. 26: 283-291.
- Li LY. 1994. Worldwide use of Trichogramma for biological control on different crops: a survey. In: Wajnberg, E. and Hassan, SA. (Eds.), Biological control with egg parasitoids, CAB International, Wallingford, UK, pp. 37–44.
- Liu N, Scott JG. 1998. Increased transcription of CYP6D1 causes cytochrome P450-mediated insecticide resistance in house fly. Insect Biochem Mol Biol. 28: 531–535.
- Lopez JD, Morrison RK. 1985. Parasitization of Heliothis spp. eggs after augmentative releases of Trichogramma pretiosum Riley. Southwestern Entomol. 8: 110–138.
- Maitra S, Dombrowski SM, Waters LC, Ganguly R. 1996. Three second chromosome-linked clustered Cyp6 genes show differential constitutive and barbitalinduced expression in DDT-resistant and susceptible strains of Drosophila melanogaster. Gene 180: 165–171.
- Morris GM, Goodsell DS, Halliday RS, Huey R, Hart WE, Belew RK, Olson AJ. 1998. Automated docking using a Lamarckian genetic algorithm and an empirical binding free energy function. J Comp Chem. 19: 1639–1662.
- Panigrahi SK. 2008. Strong and weak hydrogen bonds in protein-ligand complexes of kinases: a comparative study. Amino Acids 34: 617–633.
- Patil R, Das S, Stanley A, Yadav L, Sudhakar A, Varma AK. 2010. Optimized hydrophobic interactions and hydrogen bonding at the target-ligand interface leads the pathways of drug-designing. PLoS One 5: e12029.
- Pedra JHF, Mclntyre LM, Scharf ME, Pittendrigh BR. 2004. Genome-wide transcription profile of field and laboratory-selected dichlorodiphenyltrichloroethane (DDT)-resistant Drosophila. Proc Nat Acad Sci USA. 101: 7034–7039.
- Pittendrigh B, Aronstein K, Zinkovsky E, Andreev O, Campbell B, Daly J, Trowell S, ffrench-Constant RH. 1997. Cytochrome P450 genes from Helicoverpa armigera: expression in a pyrethroid – susceptible and – resistant strain. Insect Biochem Mol Biol. 27: 507–512.
- Ramachandran GN, Ramakrishnan C, Sasisekharan V. 1963. Stereochemistry of polypeptide chain configurations. J Mol Biol. 7: 95–99.
- Ranson H, Jensen B, Vulule JM, Wang X, Hemingway J, Collins FH. 2000a. Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids. Insect Mol Biol. 9: 491–497.
- Ranson H, Jensen B, Wang X, Prapanthadara L, Hemingway J, Collins FH. 2000b. Genetic mapping of two loci affecting DDT resistance in the malaria vector Anopheles gambiae. Insect Mol Biol. 9: 499–507.
- Rocher A, Marchand-Geneste N. 2008. Homology modeling of the Apis melifera nicotinic acetylcholine receptor (nAChR) and docking of imidacloprid and fipronil insecticides and their metabolites. SAR and QSAR in Environ Res. 19: 245–261.
- Sabourault C, Guzov VM, Koener JF, Claudianos C, Plapp Jr. FW, Feyereisen R. 2001. Overproduction of a P450 that metabolizes diazinon is linked to a loss of function in the chromosome 2 ali-esterase (MdalphaE7) gene in resistant house flies. Insect Mol Biol. 10: 609–618.
- Scott JG. 1999. Cytochromes P450 and insecticide resistance. Insect Biochem Mol Biol. 29: 757–777.
- Shen J, Wu Y. 1995. Resistance to Helicoverpa armigera to insecticides and its management. China Agricultural Press, Beijing, China, pp. 1–88.
- Solis FJ, Wets R.J-B. 1998. Minimization by random search techniques. Maths Operation Res. 6: 19–30.
- Tares S, Berge JB, Amichot M. 2000. Cloning and expression of cytochrome P450 genes belonging to the CYP4 family and to a novel Family, CYP48, in two hymenopteran insects, Trichogramma cacoeciae and Apis mellifera. Biochem Biophys Re. Commun. 268: 677–682.
- Wheelock GD, Scott JG. 1992. Anti-P450I pr antiserum inhibits specific monooxygenase activities in LPR housefly microsomes. J Exp Zool. 264: 153–158.
- Yang Y, Yue L, Chen S, Wu Y. 2008. Functional expression of Helicoverpa armigera CYP912 and CYP9A14 in Saccharomyces cerevisiae. Pesticide Biochem Physiol. 92: 101–105.
- Yano JK, Wester MR, Schoch GA, Griffin KJ, Stout CD, Johnson EF. 2004. The structure of human microsomal cytochrome P450 3A4 determined by X-ray crystallography to 2.05A° resolution. J Biol Chem. 279: 38091–38094.
- Genetic Variation in Artificially Selected Strains of the Egg Parasitoid, Trichogramma chilonis Ishii (Hymenoptera: Trichogrammatidae) Using Rapd Analysis
Abstract Views :266 |
PDF Views:148
Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore 560024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 23, No 4 (2009), Pagination: 353-359Abstract
Artificial selection is extensively used to develop pesticide resistance in natural enemies. RAPD markers were used to estimate genetic relatedness between the parent and artificially selected hybrids of the egg parasitoid Trichogramma chilonis Ishii resistant to multiple insecticides (700ppm of endosulfan, 540ppm of monocrotophos and 20ppm of fenvalerate) and high temperature (> 40°C). Highly polymorphic markers could be identified through the primers OPF-1 and OPJ-20. RAPD marker from 15 oligomers clearly outgrouped the susceptible parent from artificially selected pesticide-resistant strains. The first group comprised the susceptible strain. The second group comprised the two subclusters, the first subcluster including the strain resistant to endosulfan and fenvalerate, multiple-insecticide resistant strain (MIRS) and high temperature resistant (HTR) strain while the second subcluster consisted of monocrotophos and HTR strains. The discriminating property of RAPD markers allowed differentiation of the resistant strain from the parental susceptible strain. Artificially selected resistant strains shared relatively high similarity (61-66%) with susceptible parent strain as per Jaccard's index.Keywords
Trichogramma chilonis, RAPD, Pesticide Resistance, High Temperature, Genetic Relatednes.References
- Aljanabi, S. M., Loicacano, M. S., Lourenco, R. T., Borges,M. and Tigano, M. S. 1998. RAPD analysisrevealing polymorphism in egg parasitoid ofsoybean stink bugs (Hemiptera: Pentatomidae). Anais da Sociedade Entomologica do Brasil,27: 413-420.
- Ashok Kumar, G., Jalali, S. K., Venkatesan, T., Nagesh,M. and Lalitha, Y. 2008. Genetic improvementof egg parasitoid Trichogramma chilonis Ishii forcombined tolerance to multiple insecticides andhigh temperature. Journal of Biological Control,22: 347-356.
- Buso, G. S. C., Rangel, P. H. and Ferrira, M. E. 1998. Analysis of genetic variability of South Americanwild rice populations (Oryza glumaepatula) with isozymes and RAPD markers. Molecular Ecology,7: 107–117.
- Cenis, J. L. 1993. Indentification of four major Meloidogynespp. by random amplified polymorphic DNA(RAPD-PCR). Phytopathology, 83: 76-78.
- Dawson, I. K., Chalmers, K. J., Waugh, R. and Powell, W. 1993. Detection and analysis of genetic variationin Hordeum spontaneum populations from Israelusing RAPD markers. Molecular Ecology, 2: 151–159.
- Edwards, O. R. and Hoy, M. A. 1995. Random amplifiedpolymorphic DNA markers to monitor laboratoryselected,pesticide resistant Trioxys pallidus(Hymenoptera: Aphidiidae) after release intothree California walnut orchards. EnvironmentalEntomology, 24: 487-496.
- Hoy, M. A. 1994. Insect Molecular Genetics: Anintroduction to principles and applications, 2ndedition. Academic Press.
- Hsiao, J. Y. and Lee, S. M. 1999. Genetic diversity andmicrogeographic differentiation of Yushan cane(Yushania niitakayamensis; Poaceae) in Taiwan. Molecular Ecology, 8: 263–270.
- Huff, D. R., Peakall, R. and Smouse, P. E. 1993. RAPDvariation within and among a natural populationof outcrossing buffalo grass [Buchloë dactyloides(Nutt.) Engelm.]. Theoretical and AppliedGenetics, 86: 927–934.
- Jalali, S.K., Rabindra, R.J., Rao, N.S. and Dasan, C.B. 2003. Mass production of trichogrammatids andchrysopids. Project Directorate of BiologicalControl, Bangalore 560024, Technical BulletinNo. 33, 16pp.
- Jalali, S. K., Singh, S. P., Venkatesan, T., Murthy, K. S.,and Lalitha, Y. 2006a. Development of endosulfantolerant strain of an egg parasitoid Trichogrammachilonis Ishii (Hymenoptera: Trichogrammatidae). Indian Journal of Experimental Biology, 44: 584-590.
- Jalali, S. K., Venkatesan, T., Murthy, K. S., Rabindra, R. J.,Lalitha, Y., Udikeri, S. S., Patil, B. V., Bheemanna,M., Sreenivas, A. G., Balagurunathan, R. andYadav, D. N. 2006b. Field efficacy of multipleinsecticide tolerant strain of Trichogrammachilonis against American bollworm, Helicoverpaarmigera on cotton. Indian Journal of PlantProtection, 34: 173-180.
- Laurent, V., Wajenberg, E., Morgan, B., Schiex, T., Gaspin,C. and Vanlerberghe-Massuti, L. 1998. A compositegenetic map of parasitoid wasp Trichogrammabrassicae based on RAPD markers. Genetics,50: 275-282.
- Kumar, L. S., Sawant, A. S., Gupta, V. S. and Ranjekar, P.K. 2001. Genetic variation in Indian populationsof Scirpophaga incertulas as revealed by RAPDPCRanalysis. Biochemical Genetics, 39: 43-57.
- Martin, C., Gonzalez-Bentio, M. E. and Iriondo, J. M. 1997. Genetic diversity within and among populationsof a threatened species: Erodium paularense Fern. Gonz & Izco. Molecular Ecology, 6: 813–820.
- Pornkulwat, S., Skoda, S. R., Thomas, G. D. and Foster,J. E. 1998. Random amplified polymorphic DNAused to identify genetic variation in ecotypes ofthe European corn borer (Lepidoptera: Pyralidae). Annals of the Entomological Society of America,91:719-725.
- Rohlf, F. 1998. NTSYS-pc. Numerical taxonomy andmultivariate analysis system (version 2.02j). Exeter Software Publishers.
- Shah, R., Armstrong, K., Worner, S. P. and Chapman, R. B. 2002. Investigation of a PCR-based methodfor insecticide resistance monitoring. PakistanJournal of Biological Sciences, 5: 1070-1073.
- Subramanian, S. and Mohankumar, S. 2006. Geneticvariability of the bollworm, Helicoverpaarmigera, occurring on different host plants. 8pp. Journal of Insect Science, 6: 26.
- Vanlerberghe-Masutti F. 1994. Detection of geneticvariability in Trichogramma populations usingmolecular markers. Norwegian Journal ofAgricultural Sciences, 16: 171-176.
- Whitten, M. J. and Hoy, M. A. 1999. Enhanced biologicalcontrol through pesticide selectivity, pp. 271–296. In: Fisher T., Bellows, T. S., Caltagirone, L. E.,Dahlsten, D. L., Huffaker, C. and Gordh, G. (Eds.). Handbook of Biological Control, Academic Press.
- Williams, C. L., Goldson, S. L., Baird, D. B. and Bullock,D. W. 1994. Geographical origin of an introducedinsect pest, Listronotus bonariensis (Kuschell), determined by RAPD analysis. Heredity,72: 412-419.
- Yli-Mattila, T., Paavanen-Huhtala, S., Fenton,B. and Tuovinen, T. 2000. Species andstrain identification of the predatory miteEuseius finlandicus by RAPD-PCR andITS Sequences. Experimental and AppliedAcarology, 24: 863-880.
- Zhao, G. F., Felber, F. and Kuepfer, P. 2000. Subpopulationdifferentiation of Anthoxanthum alpinum(Poaceae) along an altitudinal gradientdetected by random amplified polymorphicDNA. Acta Phytotaxonomica Sinica,38: 64–70.
- Intraguild Predation and Biosafety of Entomopathogenic Nematode, Heterorhabditis bacteriophora Poinar et Al., and its Bacterial Symbiont, Photorhabdus luminescens, to Parasitoid, Trichogramma chilonis Ishii and Predator Chrysoperla zastrowi sillemi (Esben, Petersen)
Abstract Views :326 |
PDF Views:132
Authors
Affiliations
1 National Bureau of Agriculturally Important Insects, P.B. No. 2491, H.A. Farm Post, Hebbal, Bangalore 560 024, IN
1 National Bureau of Agriculturally Important Insects, P.B. No. 2491, H.A. Farm Post, Hebbal, Bangalore 560 024, IN
Source
Journal of Biological Control, Vol 26, No 4 (2012), Pagination: 334–340Abstract
Intraguild predation (IGP) appears to be pervasive among communities of biocontrol agents associated with nematode sharing the host with trophic interaction. Entomopathogenic nematode (Heterorhabditis bacteriophora) and its associated bacterium (Photorhabdus luminescens); an egg parasitiod, Trichogramma chilonis and a predator Chrysoperla zastrowi sillemi were selected for present study. There was no adverse effect of H. bacteriophora and P. luminescens observed on adult emergence of T. chilonis. Microscopic examination of eggs, larvae and adults of T. chilonis and C. z. sillemi treated with H. bacteriophora, P. luminescens and cell-free culture filtrates of P. luminescens, exhibited no deformity, discoloration or infection of organisms. Similarly, H. bacteriophora, P. luminescens or the cell-free culture filtrates exhibited no adverse activity on egg hatching or larvae of C. z. sillemi indicating that there was no intraguild competition under artificial epiphytotic conditions between the organisms under report, viz., H. bacteriophora, its associated bacterium (P. luminescens), T. chilonis and C. z. sillemi.Keywords
Intraguild Predation, Entomopathogenic nematode, Heterorhabditis bacteriophora, Photorhabdus luminescens, Trichogramma chilonis, Chrysoperla zastrowi sillemi.References
- Akhurst RJ. 1980. Morphological and functional dimorphism in Xenorhabdus spp., bacteria symbiotically – associated with the insect pathogenic nematodes, Neoaplectana and Heterorhabditis. J Gene Microbiol. 121: 303–309.
- Boemare N, Laumond C Mauléon H. 1996. The entomopathogenic nematode-bacterium complex: Biology, life cycle and vertebrate safety. Bio Sci Tech. 6: 333–345.
- Ehlers RU. 1996. Current and future use of nematodes in biocontrol: Practice and commercial aspects with regard to regulatory policy issues Bio Sci Tech. 6: 303–316.
- Elzen GW, Elzen PJ, King EG. 1998. Laboratory toxicity of insecticide residues to Orius insidiosus, Geocoris punctipes, Hippodamia convergens and Chrysoperla carnea. Southwest Entomol. 23: 335–342.
- Fischer-Le Saux M, Viallard V, Brunel B, Normand P, Boemare N. 1999. Polyphasic classification of the genus Photorhabdus and proposal of new taxa. P. luminescens subsp. luminescens subsp nov., P. luminescens subsp. akhurstii subsp. nov., P. temperata subsp. temperata subsp. nov. and P. asymbiotica sp. nov. Int J Syst Bacteriol. 49: 1645–1656.
- Gaugler R, Wilson M, Shearer P. 1997. Field release and environmental fate of a transgenic entomo-pathogenic nematode. Biol. Control. 9: 75–80.
- Georgis R, Kaya HK, Gaugler R. 1991. Effect of steinernematid and heterorhabditid nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) on nontarget arthropods Environ Entomol. 20: 815–822.
- Haag KH, Boucias DG. 1991. Infectivity of insect pathogens against Neochetina eichhorniae, a biological control agent of water hyacinth. Fla Entomol. 74: 128–133.
- Hassan SA. 1980. [A reproducible laboratory procedure for testing the persistence of the side-effect of pesticides on egg parasites of the genus Trichogramma (Hymenoptera: Trichogrammatidae.] Zeitschrift for Angewandie Entomologie 39: 282–289.
- Hassan SA. 1985. Standard methods to test the side-effects of pesticides on natural enemies of insects and mites developed by the IOBC/WPRS Working group Pesticides and beneficial organisms. Bulletin OEPP/ EPPO Bull. 15: 214–255.
- Jalali SK, Rabindra RJ, Rao NS, Dasan CB. 2003. Mass production of Trichogrammatids and chrysopids. Technical Bulletin No. 33, Project Directorate of Biological Control, H.A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, India, pp. 16.
- Jansson RK. 1993. Introduction of exotic entomopathogenic nematodes (Rhabditida: Heterorhabditidae and Steinernematidae) for biological control of insects: Potential and problems. Fla Entomol. 76: 82–96.
- Jaworska M, Ropek D, Prusznski S. 1995. The influence of entomopathogenic nematodes on insect natural enemies – Wplyw owadobojczych nicieni na wrogow naturalnych owadow Materialy Sesji Instytutu Ochrony Roslin. 35: 434–436.
- Kaya HK. 1978. Interaction between Neoaplectana carpocapsae (Nematoda: Steinernematidae) and Apanteles militaris (Hymenoptera: Braconidae), parasitoid of the armyworm, Pseudaletia unipuncta. J. Inv. Pathol. 31: 358–364.
- Kaya HK. 1984. Effect of the entomogenous nematode Neoaplectana carpocapsae on the tachinid parasite Compsilura concinnata (Diptera: Tachinidae). J Nematol. 16: 9–13.
- Li, Li-Yung. 1994. World-wide use of Trichogramma for biological control on different crops. A survey. In Biological Control with egg parasitoids (Eds: Wajnberg, E. and Hassan, SA). CAB International, Oxon, UK. Pp 37–53.
- Mohan S, Sabir N. 2005. Biosafety concern on the use of Photorhbdus luminescens as biopesticide: experimental evidence of mortality in egg parasitoid Trichogramma spp. Curr Sci. 89: 1268–1272.
- Mohan S, Raman R, Gaur HS. 2003. Foliar application of Photorahabdus luminiscens symbiotic bacteria from entomopathogenic nematode, Heterorhabditis indica to kill cabbage butter fly, Pieris brassicae. Curr. Sci. 84: 1397.
- Mohan S, Sirohi A, Gaur HS. 2004. Successful management of mango mealybug, Drosicha mangiferae by Photorhabdus luminescens, a symbiotic bacterium from entomopathogenic nematode Heteror-habditis indica. Int J Nematol. 14: 195–198.
- Mrácek Z, Spitzer K. 1983. Interaction of the predators and parasitoids of the sawfly, Cephalcia abietis (Pamphilidae: Hymenoptera) with its nematode Steinernema kraussei J Inv Pathol. 42: 397–399.
- Razek-Abdel AS. 2003. Pathogenic effects of Xenorhabdus nematophilus and Photorhabdus luminescens (Enterobacteriaceae) against pupae of the diamondback moth, Plutella xylostella (L.). J Pest Sci. 76: 108–111.
- Richardson PN. 1996. British and European legislation regulating rhabditid nematodes Bio Sci Tech. 6: 449–463.
- Rizvi SA, Hennessey R, Knott D. 1996. Legislation on the introduction of exotic nematodes in the US. Bio. Sci. Techo. 6: 477–480.
- Rosenheim JA, Kaya HK, Ehler LE, Marois JJ, Jaffee BA. 1995. Intraguild predation among biological-control agents: Theory and Evidence. Biol. Control. 5: 303–335.
- Smits PH. 1996. Post-application persistence of entomopathogenic nematodes. Bio Sci Tech. 6: 379–387.
- Screening and in Vitro Evaluation of Native Pseudomonas Spp., against Nematode Pathogens and Soil Borne Fungal Pathogens
Abstract Views :277 |
PDF Views:153
Authors
Affiliations
1 Central Plantation Crops Research Institute, Kasargod 671 124, Kerala, IN
2 National Bureau of Agriculturally Important Insects (NBAII), Bangalore 560 024, IN
1 Central Plantation Crops Research Institute, Kasargod 671 124, Kerala, IN
2 National Bureau of Agriculturally Important Insects (NBAII), Bangalore 560 024, IN
Source
Journal of Biological Control, Vol 27, No 4 (2013), Pagination: 305–311Abstract
The objective of this study was to assess the efficacy of native Pseudomonas spp., against ischolar_main-knot nematode, Meloidogyne incognita and other soil borne fungal pathogens such as Fusarium oxysporum f. sp. Lycopersici and Sclerotium rolfsii. The eggs and second stage juveniles (J2) of M. incognita were exposed to each isolates of Pseudomonas spp., by diluting the standard culture filtrate to fifty percent and to undiluted culture filtrate (100%). Four isolates of Pseudomonas spp. (CRS3, CRS6, and CRS8 and CRS10) significantly induced inhibition of egg hatching and mortality of M. incognita juveniles. The per cent mortality was proportional to the concentration of culture filtrate and the duration of exposure period. The highest percentage of inhibition of egg hatching was recorded for CRS3 while mortality of second stage juveniles was found in the case of CRS10 in undiluted culture filtrate. The CRS6 caused 48% inhibition of Sclerotium rolfsii while CRS8 caused 58% inhibition of Fusarium oxysporum f. sp. lycopersici. Since meloidogyne infection can predispose plants to plant pathogens, these isolates show promise for management of nematode and disease complex of vegetable crops.Keywords
Culture Filtrate, Egg Hatching, Meloidogyne Incognita, Pseudomonas Spp., Root-knot Nematode and Soil Borne Fungi.References
- Anonymous 1957. Manual of microbiological methods. Society of American Bacteriologists, McGraw Hill Book Company, Inc., New York, 315 pp.
- Ashraf MS, Khan TA. 2010. Integrated approach for the management of Meloidogyne javanica on eggplant using oil cakes and bio-control agents. Arch. Phytopathology. Pl Prot. 43: 609–614.
- Becker JO, Zavaleta-Mejia, Colbert SF, Schroth MN, Weinhold AR, Hancock JG, Van Gundy SD. 1988.
- Effects of rhizobacteria on ischolar_main-knot nematodes and gall formation. Phytopathol 78: 1466–1469.
- Buchanan RE, Gibbons NE. 1974. Bergey’s Manual of Determinative Bacteriology. Baltimore, Williams & Wilkins, 8th edition, pp. 13–45.
- Carter CC. 1985. Literature search: host range of Meloidogyne hapla. Internatl Nematol Network Newslett. 2: 16–24.
- Cayrol JC, Djian C, Pijarowski I. 1989. Studies on the nematicidal properties of the culture filtrate of the nematophagous fungus Paecilomyces lilacinus. Revue de Nematologie 12: 331–336.
- Dawar S, Wahab S, Tariq M, Zaki MJ. 2010. Applications of Bacillus species in the control of ischolar_main-rot disease of crop plants. Arch Phytopathol Pl Prot. 43 (4): 412–418.
- Gokte N, Swarup G. 1988. On the potential of some bacterial biocides against ischolar_main-knot and cyst nematodes. Ind J Nematol. 18: 152–153.
- Goswami BK, Bhattacharya Chaitali, Singh Neetu, Paul MP Reject.2012. An IPM package for the management of soil borne fungal and ischolar_main-knot nematode diseases on tomato and okra. Pesticide Res J. 24(1): 91–95.
- Hanna AL, Raid FW, Tawfik AE. 1999. Efficacy of antagonistic rhizobacteria on the control of ischolar_main-knot nematode, Meloidogyne incognita in tomato plants. Egyptian J Agri Res. 77: 1467–1476.
- Hartman KM, Sasser JN. 1985. Identification of Meloidogyne species on the basis of differential host test and perineal pattern morphology. pp. 69–77. In: Barker KR, Carter, CC, Sasser JN (ed.). An Advanced Treatise on Meloidogyne. Vol II, methodology, North Carolina State University Graphics, Raleigh, NC, USA. Hussey RS, Barker KR. 1973. A comparison of methods of collecting inocula of Meloidogyne spp., including a new technique. Pl Dis Rep. 61: 328–331.
- Ioannis K, Vagelas, Baerbark Pembroke, Simon R, Gowen and Keith G. Davies. 2007. The control of ischolar_main-knot nematodes (Meloidogyne spp.) by Pseudomonas oryzihabitans and its immunological detection on tomato ischolar_mains. Nematol. 9 (3): 363–370. Johnsson L, Hökeberg M, Gerhardson B. 1998. Performance of the Pseudomonas chlororaphis, biocontrol agent MA 342 against seed-borne diseases in field experiments. European J Pl Pathol. 104: 701–711.
- Khan Z, Park SD, Shin SY, Bae SG, Yeon IK, Seo YJ. 2005. Management of Meloidogyne incognita on tomato by ischolar_main dip treatment in culture filtrate of the blue green alga, Microcoleus vaginatus. Biores Technol. 96: 1338–1341.
- Kienwnick S, Sikora RA. 2006. Biological control of the ischolar_main-knot nematode, Meloidogyne incognita by Paecilomyces lilacinus strain 251. Biol Cont. 38: 179– 187.
- King EO, Ward MK, Raney DE. 1954. Two simple media for the demonstration of pyocyanin and fluorescin. J Lab Clin Med. 11: 441–449.
- Knight KWL, Barber CJ, Page GD. 1997. Plant-parasitic nematodes of New Zealand recorded by host association. J Nematol. 29: 640–656.
- Kuruganti K. 2005. Effects of pesticide exposure on developmental task performance in Indian children, Youth and Environ. 15 (1): 83–114.
- Li bin, Xie Guan-lin, Soad A, Coosemans J. 2005. Suppression of Meloidogyne javanica by antagonistic and plant growth promoting rhizobacteria. J Zhejjang. 496–501.
- Mohamed Hashem, Kamal Abo-Elyousr A. 2011. Management of ischolar_main-knot, Meloidogyne incognita on tomato with combinations of different bio-control organisms. Crop Prot. 30: 1–8.
- Mujeebur Rahman Khan, Shaharana Majid, Fayaz A, Mohidin, Nibilah Khan. 2011. A new bioprocess to produce low cost powder formulations of bio-control bacteria and fungi to control fusarial wilt and ischolar_mainknot nematodes of pulses. Biol Cont. 59: 130–140.
- Nash SN, Snyder WC. 1962. Quantitative estimation of plate counts of propagules of the bean ischolar_main rot Fusarium in field soils. Phytopathol. 52: 567–572.
- Oostendorp M, Sikora RA. 1990. In vitro inter-relationships between rhizosphere bacteria and Heterodera schachtii. Rev de Nematol. 13: 269–274.
- Reddy Parvatha P.1986. Chemical control of plant parasitic nematodes infecting citrus and grapevine. Ind J Hort 43: 303–305.
- Regina MDGC, DeSouza IS, Belarmino LC. 1998. Nematicidal activity of Bacillus spp. strains on juveniles of Meloidogyne javanica. Nemat Brasiliera 22: 12–21.
- Sasser JN. 1979. Economic importance of Meliodogyne in tropical countries, pp. 359–374. In: Lamberti, F., Tylor, C. E. (Eds.) Root-knot nematodes (Meloidogyne species) Systematics, Biology & Control. Academics Press. London.
- Shankra T, Pavaraj M, Umamaheswari Prabhu D, Baskaran S. 2011. Effect of Pseudomonas aeruginosa on the ischolar_main-knot nematode, Meloidogyne incognita infecting tomato, Lycopersicum esculentum. A J Ent. 4 (3): 114–117.
- Sharma SB, Rupela OP, Ansari MA, Gopalkrishnan S. 1998. Suppression of Meloidogyne javanica egg hatch by on isolate of Pseudomonas striata. In: III International Symposium of Afro-Asian Society of Nematologists, 16-19 April 1998, Coimbatore, India.
- Siddiqui ZA, Ehteshamul-Haque S, Shukat SS. 2001. Use of rhizobacteria in the control of ischolar_main rot- ischolar_main-knot disease complex of mungbean. J Phytopathol. 149: 337–346.
- Siddiqui I, Shukat S. 2003. Suppression of ischolar_main diseases by Pseudomonas fluorescenes CHAO in tomato: Importance of bacterial secondary metabolities 2, 4-diacetylphloroglucinol. Soil Biol Biochem. 35: 1 615 – 1623.
- Siddiqui ZA, Mahmood I. 1999. Role of bacteria in the management of plant parasitic nematodes: a review. Bio-resource Tech. 69: 167–179.
- Vagelas IK. 2002. Efficacy of Pseudomonas oryzihabitans as bio-control agent of ischolar_main pathogens. Ph.D. Thesis, Department of Agriculture, University of Reading, UK.
- Vagelas IK, Gravanis FT, Gowen SR. 2003. Control of Fusarium oxysporum and Meloidogyne spp. with Pseudomonas oryzihabitans. In: Proceedings of the BCPC International Congress – Crop Science and Technology. Vol. 1. Glasgow, UK., 419–424.
- Genetic Improvement of Egg Parasitoid Trichogramma chilonis Ishii for Combined Tolerance to Multiple Insecticides and High Temperature
Abstract Views :224 |
PDF Views:114
Authors
Affiliations
1 Biological Control, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
2 Biological Control, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
3 Biological Control, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
1 Biological Control, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
2 Biological Control, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
3 Biological Control, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Bangalore, 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 22, No 2 (2008), Pagination: 347-356Abstract
A strain of Trichogramma chilonis Ishii, an effective parasitoid of lepidopteran pests was developed for tolerance to three major groups of insecticides, i. e., endosulfan (organochlorine), monocrotophos (organophosphate) and fenvalerate (synthetic pyrethroid) and to high temperature (32-38°C) through selection. After 81 generations of selection, there was an increase in parasitism from 35% to 90-95% and decrease in mortality from 100% to 57-70% after 6h of constant exposure to three insecticides and high temperature. Interestingly 46.8 and 2.9 fold increase in tolerance was observed in males compared to 18.5 and 1.3 fold increase in females of MITT (multiple-insecticide and temperature tolerant) strain when exposed to endosulfan and fenvalerate sprays, respectively, while in case of monocrotophos, females and males were 465-fold and 25-fold more tolerant, respectively, than susceptible strain. At higher temperatures of 40 and 45°C, the per cent mortality of tolerant adults was 0.0 and 9.2%, respectively, compared to 59.7 and 96.1% in susceptible population after 6h of exposure at these temperatures. At high variable temperature (32-38°C), per cent mortality in MITT was 57.1 compared to 98.5% in susceptible population after 24h of constant exposure. The mean per cent parasitism at 32°C, 36°C, 38°C and at variable temperature of 32-38°C was significantly higher in tolerant strain (46.7, 45.0, 18.3 and 63.3%) compared to susceptible strain (54.0, 6.7, 0.0 and 0.0%), respectively. These studies suggest that the improved strain of T. chilonis will provide effective control of the pest even at harsh climatic conditions and under high insecticide pressure in different economically important crops.Keywords
Egg Parasitoid, Genetic Improvement, High Temperature Tolerance, Multiple Insecticide Tolerance, Trichogramma chilonis.- Biochemical and Molecular Profiling of Indigenous Xenorhabdus Isolates Associated with Steinernema Spp
Abstract Views :254 |
PDF Views:132
Authors
H. S. Vidya
1,
M. Nagesh
1
Affiliations
1 Project Directorate of Biological Control, Post Box No.2491, H. A. Farm Post, Bellary Road, Bangalore - 560024, Karnataka, IN
1 Project Directorate of Biological Control, Post Box No.2491, H. A. Farm Post, Bellary Road, Bangalore - 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 21, No 2 (2007), Pagination: 203-209Abstract
Bacterial symbionts were obtained from entomopathogenic nematodes Steinernema carpocapsae, S, riobrave, S. feltiae and S. tami, and identified as belonging to Xenorhabdus species by subjecting to biochemical characterization. The Xenorhabdus isolates were further characterized for their interspecific variation by protein profiling and RFLP analysis of 16S rDNA. The protein profiles recorded discernible differences with protein distribution ranging from 97Kda to 14Kda, but most of the fragments were common to all isolates. RFLP analysis of 16S rDNA of the isolates using eight restriction enzymes showed the distinctness of isolates and based on the restriction enzyme patterns, the isolates were grouped into two clusters. The study showed that a combination of biochemical and molecular techniques could be used for the identification and characterization of Xenorhabdus isolates.Keywords
Indigenous Isolates, PCR-RFLP, Steinernema, Xenorhabdus, 16S rDNA.- Partial Characterization of Novel Nematicidal Toxins from Bacillus cereus Frankland and Frankland 1887 and their Effect on Root-Knot Nematode, Meloidogyne incognita (Kofoid & White) Chitwood
Abstract Views :226 |
PDF Views:133
Authors
Affiliations
1 Biological Control, P. B. Ho. 2491, H. A. Farm Post, Bellary Road. Hebbal, Bangalore, 560 024, IN
2 Division of Entomology and Nematology, Indian Institute of Horticultural Research, Hessaraghatta Lake P. O., Bangalore, 560 089, Karnataka, IN
3 Monsanto Research Centre, #44/2A. Vasants' Business Park, Bellary Road, Bangalore, 560 092, IN
1 Biological Control, P. B. Ho. 2491, H. A. Farm Post, Bellary Road. Hebbal, Bangalore, 560 024, IN
2 Division of Entomology and Nematology, Indian Institute of Horticultural Research, Hessaraghatta Lake P. O., Bangalore, 560 089, Karnataka, IN
3 Monsanto Research Centre, #44/2A. Vasants' Business Park, Bellary Road, Bangalore, 560 092, IN
Source
Journal of Biological Control, Vol 19, No 1 (2005), Pagination: 65-70Abstract
Bacillus cereus isolated from the egg masses of ischolar_main-knot nematode, Meloidogyne incognita from tomato rhizosphere was grown in casein-peptone soy meal peptone (CASO) broth for partial characterization of toxins from cell-free filtrates. Cell-free culture filtrates of B. cereus at 72 hours of growth and beyond, reduced egg hatching (90%) and caused 100 per cent mortality of juveniles in 4 hours of exposure, which coincided with the post-sporulation phase (72h) of the bacterial growth indicating profound toxicity during and after the sporulation phase. The native PAGE electrophoresis of the partially purified cell-free culture filtrates showed that 3 bands of ≈ 15, 40 and 60 kDa appeared in 72, 84 and 96 hours of growth, respectively, coinciding with post-sporulation phase of the bacterium.Keywords
Bacillus cereus, Meloidogyne incognita, Nematicidal Toxins, Partial Characterization.- Influence of Laboratory Culturing of Paecilomyces lilacinus (Thomson) Sams, and Pochonia chlamydosporiazare et Al. on Spore Viability and Infeetivity against Meloidogyne incognita Chitwood Eggs
Abstract Views :229 |
PDF Views:147
Authors
Affiliations
1 Project Directorate of Biological Control (lCAR), Post Bag No. 2491. H. A. Farm Post. Bellary Road, Bangalore 560 024, Karnataka, IN
1 Project Directorate of Biological Control (lCAR), Post Bag No. 2491. H. A. Farm Post. Bellary Road, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 19, No 2 (2005), Pagination: 187-191Abstract
Two native isolates each of Paecilomyces lilacinus (Thomson) Sams. (PDBC PL55 and PDBC PLS8) and Pochonia chlamydosporia Zare et al. (PDBC VC56 and PDBC VC57) were subjected to repeated sub-culturing for ten generations at 28 ± 1°C with intervening resting periods in refrigerated conditions to evaluate the effect on their spore viability and infeetivity. Significant decrease in spore viability was noticed from generation IX in case of P. lilacinus isolates (PDBC PLSS and 58), while, P. chlamydosporia (PDBC VCS6 and 57) isolates recorded significant reduction in spore viability from generation VII. The infeetivity of the P. lilacinus and P. chlamydosporia isolates under study were on par with each other in ail the generations, which recorded significant reduction in tenth generation. Between the spore viability and infectivity of the isolates under report, spore viability was more influenced by repeated sub-culturing than infeetivity.Keywords
Infectivity, Meloidogyne incognita, Paecilomyces lilacinus, Pochonia chlamydosporia, Spore Viability, Sub-Culturing.- Management of Root-knot Nematode, Meloidogyne incognita (kofoid & White) Chitwood in Chrysanthemum Using Paecilomyces lilacinus (Thom) Samson in Combination with Neem Cake
Abstract Views :222 |
PDF Views:149
Authors
Affiliations
1 Project Directorate of Biological Control (ICAR) P.B. No. 2491, H.A. Farm P.O., Bellary Road Bangalore 560024, Karnataka, IN
1 Project Directorate of Biological Control (ICAR) P.B. No. 2491, H.A. Farm P.O., Bellary Road Bangalore 560024, Karnataka, IN
Source
Journal of Biological Control, Vol 17, No 2 (2003), Pagination: 125-131Abstract
Talc and pesta granule formulations of P. lilacinus spores, obtained from solid and liquid media, at 12, 10, 8 and 6 per cent moisture levels were evaluated for their spore viability and tbeir field efficacy, singly and in combination with neem cake against ischolar_main-knot nematode, Meloidogyne incognita in the farmer's field. The preparation of P. lilacinus used in the field consisted mainly of spores. The fungus was cultured under two sets of defined conditions to produce aerial spores, i. e., cultured on sorghum grains, and submerged spores, grown in a liquid medium. Aerial spores were more robust (96, 87, 80 and 60% viability at 6, 8, 10 and 12% moisture levels, respectively, in talc formulation from sorghum grains), compared to submerged spores (83, 72,64 and 54% viability at 6, 8, 10 and 12% moisture levels, respectively, in talc formulation from liquid broth), over a period of 60 days after formulation. Talc and pesta granules of P. lilacinus, applied at 2 doses, 10 and 15 kg/ha to the ischolar_main-knot nematode infested chrysanthemum field correspondingly reduced ischolar_main gall index to 1.4-2.7, nematode multiplication rate to 1.4-2.12, and enhanced floral yield by 12 per cent depending on the dose of formulation. Further, combined use of these formulations with neem cake enhanced fungal propagules in rhizosphere to 700-1070, fungal infectivity to 32- 52 per cent and chrysanthemum flower yield (by 23-28 %) depending on the dose of formulation.Keywords
Chrysanthemum, Field Evaluation, Formulations, Meloidogyne incognita, Moisture Levels, Paecilomyces lilacinus.- Pathogenicity of Selected Antagonistic Soil Fungi on Meloidogyne incognita (Kofoid & White) Eggs and Egg Masses under in Vitro and in Vivo Conditions
Abstract Views :239 |
PDF Views:140
Authors
Affiliations
1 Division of Entomology and Nematology Indian Institute of Horticultural Research Hessaraghatta Lake P.O., Bangalore 560089, Karnataka, IN
1 Division of Entomology and Nematology Indian Institute of Horticultural Research Hessaraghatta Lake P.O., Bangalore 560089, Karnataka, IN
Source
Journal of Biological Control, Vol 15, No 1 (2001), Pagination: 63- 68Abstract
Four indigenous antagonistic fungi belonging to 3 species, Gliocladium virens Miller Giddens & Foster, Paecilomyces lilacinus (Thon) Samson, Trichoderina harzianum Rifai and T. viride Pers. Ex S. F. Gray evaluated for their pathogenicity to eggs and egg masses of Meloidogyne incognita, in vitro through the Petri-dish assay and in vivo under glasshouse conditions were found pathogenic. Under both the conditions, maximum egg mass and egg parasitization was observed to be by P. lilacinus followed by T. viride, G. virens and T. harizianum. There was a general decline in parasitization by these fungi, under in vivo conditions compared to that under in vitro conditions. Fungal colonization in galled ischolar_mains was higher than in healthy ischolar_mains. Comparatively T. viride and G. virens recorded higher ischolar_main colonization closely followed by P. lilacinus. Among the four fungi, P. lilacinus recorded consistently higher pathogenicity against ischolar_main-knot nematodes both in vitro and in vivo conditions, indicating that P. lilacinus was more efficient against ischolar_main-knot nematodes compared to other species.Keywords
Antagonistic Fungi, Meloidogyne incognita, Pathogenicity.- Effect of Different Storage Temperature Regimes on Spore Viability of Paecilomyces lilacinus (Thom.) Samson in some Formulations
Abstract Views :206 |
PDF Views:131
Authors
Affiliations
1 Division of Entomology and Nematology Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore 560089, Karnataka, IN
1 Division of Entomology and Nematology Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore 560089, Karnataka, IN
Source
Journal of Biological Control, Vol 15, No 1 (2001), Pagination: 73-75Abstract
A laboratory experiment was carried out to identify optimum formulation medium and temperature for storage of Paecilomyces lilacinus based on spore viability. Paecilomyces lilacinus spores were produced on PDA, paddy grain, sorghum grain and tapioca broth, formulated on coconut coir pith, talc and vermiculite and stored at 0, 15 and 27 ± 1°C for 150 days, with P. lilacinus spores stored at -20°C on sterile silica grain as control. In general, the spore viability in grain based formulations (paddy and sorgbum) declined more rapidly than on tapioca broth based formulations. The spores stored at -20°C on sterile silica grain recorded 98 per cent viability even at 150th day of storage. Spores formulated on tapioca broth with talc, vermiculite or coir pith as inert base, and on paddy grain with talc as inert base, exhibited more tban 80 per cent viability at 150 days of storage at ooe temperature which declined to ≥60 per cent at 27 ± 1°C after 150 days of storage.Keywords
Formulations, Paecilomyces lilacinus, Spore Viability, Storage Temperatures.- A Modified Method for Mass Multiplication of Pasteuria penetrans (thorne) Sayre & starr
Abstract Views :230 |
PDF Views:130
Authors
Affiliations
1 Division of Entomology and Nematology, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore 560089, Karnataka, IN
1 Division of Entomology and Nematology, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore 560089, Karnataka, IN
Source
Journal of Biological Control, Vol 12, No 1 (1998), Pagination: 63-66Abstract
A simple method to mass multiply Pasteuria penetrans (Thorne) Sayre&Starr an obligate parasite of plant parasitic nematodes, was worked out by modifying the method defined by Stirling and Wachtel (1980). With the increase in seed rate/pot, there was an increase in ischolar_main content/pot and number of Meloidogyne incognita (Kofoide&White) Chitwood females which in turn contributed to an increase in number of infected females and spore number/g ischolar_main. This method not only enhanced spore production efficiency/pot but also reduced the inherent disadvantage of maintaining and handling a large number of plants under individual pot (in vivo) conditions.Keywords
Eggplant, Mass Multiplication, Meloidogyne incognita, Pasteuria penetrans.- Comparative Efficacy of Paecilomyces lilacinus (Thom.) Samson and Verticillium lecanii (A. Zimmerman) Viegas in Combination with Botanicals against Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949 Infecting Crossandra
Abstract Views :220 |
PDF Views:113
Authors
Affiliations
1 Division of Entomology and Nematology, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore-560 089, IN
1 Division of Entomology and Nematology, Indian Institute of Horticultural Research, Hessaraghatta Lake P.O., Bangalore-560 089, IN
Source
Journal of Biological Control, Vol 9, No 2 (1995), Pagination: 109-112Abstract
Investigations were conducted on the management of the ischolar_main-knot nematode, Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949 infecting crossandra, by integrating the use of two biocontrol agents viz., Paecilomyces lilacinus (Thom.) Samson and Verticillium lecanii (A. Zimmerman) Viegas with leaf extracts of castor and neem (5% each) as bare ischolar_main dip and soil drench. Combinations of V. lecanii and P. lilacinus (2 × 104 spores/ml each) with 5% neem leaf extract resulted in significantly higher plant growth parameters and flower yield. Root gall index was least under V. lecanii plus neem leaf extract (5%) combination followed by V. lecanii plus castor leaf extract (5%). The per cent egg and egg mass parasitization were higher in P. lilacinus than in V. lecanii when they were employed singly without any leaf extract combinations. However, integration with castor leaf extract enhanced the fungal parasitization by P. lilacinus more than that by V. lecanii. Integration with neem leaf extract improved the parasitization by V. lecanii more than that by P. lilacinus.Keywords
Bare Root Dip, Castor, Crossandra, Leaf Extracts, Meloidogyne incognita, Neem, Paecilomyces lilacinus, Soil Drench, Verticillium lecanii.- Variability in Foraging Behaviour, Thermal Requirement and Virulence of Entomopathogenic Nematodes against Sod Webworm, Herpetogramma phaeopteralis Guenee (Lepidoptera: Crambidae)
Abstract Views :297 |
PDF Views:153
Authors
M. Nagesh
1,
M. Balachander
1,
T. M. Shivalingaswamy
1,
J. Patil
1,
A. N. Shylesha
1,
A. Raghavendra
1
Affiliations
1 ICAR-National Bureau of Agricultural Insect Resources, P. B. No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore − 560 024, Karnataka, IN
1 ICAR-National Bureau of Agricultural Insect Resources, P. B. No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore − 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 33, No 1 (2019), Pagination: 36-47Abstract
Variability in virulence among entomopathogenic nematodes, Heterorhabditis indica, H. bacteriophora and Steinernema abbasi, was studied for lethality, foraging behaviour in response to host volatiles, thermal requirements (degree-days), recyclability, persistence and field efficacy against Turfgrass Sod Webworm (TSW), Herpetogramma phaeopteralis. Comparatively, lethal concentration and time were lowest for H. indica against TSW. Recyclability of EPN ranged from 3.42 × 105 to 4.23 × 105 IJs g1 of TSW. H. bacteriophora recorded highest movement rate on agar (0.38–0.78cm) and sand-agar (0.45–0.56cm), followed by S. abbasi, and H. indica. Responding to TSW volatiles, S. abbasi recorded maximum movement, H. bacteriophora, moderate, and H. indica, lowest. Heterorhabditis indica (with nictitation); S. abbasi and H. bacteriophora (without nictitation) were ambusher and cruiser, respectively. S. abbasi preferred warmer temperatures (30–33°C), H. bacteriophora, moderate (24–27°C), and H. indica, a wider range (24–30°C), for virulence based on thermal requirement. In field, EPNs were comparable to chlorpyriphos against TSW. We demonstrated the complementarity of thermal preferences of EPNs and insect pest was critical besides attributes like foraging behaviour, recyclability, persistence, and lethality values for their success in the field.Keywords
Degree-Days, Foraging, Herpetogramma phaeopteralis, Heterorhabditis indica, H. bacteriophora, Sod Webworm, Steinernema abbasi, Thermal Requirement, Turfgrass, Virulence, Variability.References
- Abbott WS. 1925. A method of computing the effectiveness of an insecticide. J Econ Entomol. 18: 265−267. https:// doi.org/10.1093/jee/18.2.265a.
- Bélair G, Koppenhöfer AM, Dionne J, Simard L. 2010. Current and potential use of pathogens in the management of turfgrass insects as affected by new pesticide regulations in North America. Intl J Pest Manage. 56: 51−60. https:// doi.org/10.1080/09670870903076012.
- Campbell JF, Gaugler R. 1993. Nictation behavior and its ecologicalimplications in the host search strategies of entomopathogenic nematodes (Heterorhabditidae and Steinernematidae). Behaviour 126: 155−169. https:// doi.org/10.1163/156853993X00092.
- Campbell JF, Gaugler R. 1997. Inter-specific variation in entomopathogenicnematode foraging strategy: dichotomy or variation along a continuum. Fundam Appl Nematol. 20: 393−398.
- Dhillon MK, Sharma HC. 2009. Temperature influences the performance and effectiveness of field and laboratory strains of the ichneumonid parasitoid, Campoletis chlorideae. BioControl 54: 743−750. https://doi.org/10.1007/s10526-009-9225-x.
- Ebssa L, Koppenhofer AM. 2011. Efficacy and persistence of entomopathogenic nematodes for black cutworm control in turfgrass. Biocontrol SciTechn. 21: 779−796. https://doi.org/10.1080/09583157.2011.584610.
- Glazer I, Lewis EE. 2000. Bioassays for entomopathogenic nematodes. pp. 229−247. In: Navon A, Ascher KRS (Eds.). Bioassays of Entomopathogenic Microbes and Nematodes. Wallingford, UK: CABI Publishing. https://doi.org/10.1079/9780851994222.0229.
- Grewal PS, Koppenhofer AM, Choo HY. 2005. Lawn, turfgrass, and pasture applications. Pp.115−146. In: Grewal PS, Ehlers RU, and Shapiro-Ilan DI, (Eds.). Nematodes as biocontrol agents. Wallingford: CABI Publishing. https://doi.org/10.1079/9780851990170.0115.
- Haydu JJ, Hodges AW, Hall CR. 2006. Economic impacts of the turfgrass and lawncare industry in the United States. University of Florida, IFAS, EDIS document FE 632.
- Hill MP, Malan AP, Terblanche JS. 2015. Divergent thermal specialisation of two South African entomopathogenic nematodes. Peer J. 3: e1023. http://doi.org/10.7717/peerj.1023. https://doi.org/10.7717/peerj.1023.
- Kaya HK. 1990. Soil ecology. Pp. 93−115. In: Gaugler R and Kaya HK, (Eds.). Entomopathogenic nematodes in biological control. Boca Raton: CRC Press.
- Klein MG, Grewal PS, Jackson TA, Koppenhofer AM. 2007. Lawn, turf and grassland pests.Pp. 655−675. In: Lacey LA and Kaya HK (Eds.), Field Manual of Techniques in Invertebrate Pathology: Application and evaluation of pathogens for control of insects and other invertebrate pests. Second Edition. Dordrecht: Springer. https://doi.org/10.1007/978-1-4020-5933-9_32.
- Koppenhofer AM, Grewal PS, Fuzy EM. 2006. Virulence of the entomopathogenic nematodes, Heterorhabditis bacteriophora, Heterorhabditis zealandica, and Steinernema scarabaei against five white grub species (Coleoptera: Scarabaeidae) of economic importance in turfgrass in North America. Biol Control 38: 397−404. https://doi.org/10.1016/j.biocontrol.2005.12.013.
- Kurtz B, Toepfer S, Ehlers RU, Kuhlmann U. 2007, Assessment of establishment and persistence of entomopathogenic nematodes for biological control of western corn ischolar_mainworm. J Appl Entomol. 131: 420−425. doi: 10.1111/j.1439-0418.2007.01202. https://doi.org/10.1111/j.1439-0418.2007.01202.x.
- Lacey AL, Georgis R. 2012. Entomopathogenic nematodes for control of insect pests above and below ground with comments on commercial production. J Nematol. 44(2): 218−225.
- Lewis EE. 2002. Behavioral Ecology. Pp. 205−224. In: Gaugler R, (Ed.), Entomopathogenic Nematology. Wallingford: CABI Publishing. https://doi.org/10.1079/9780851995670.0205.
- Lewis EE, Gaugler R, Harrison R. 1992. Entomopathogenic nematodehost finding: response to host contact cues by cruise and ambush foragers. Parasitology 105: 309−315. https://doi.org/10.1017/S0031182000074230.
- Lewis EE.Gaugler R, Harrison R. 1993. Response of cruiser and ambusher entomopathogenic nematodes (Steinernematidae) in host volatile cues. Can J Zool. 71: 765−769. https://doi.org/10.1139/z93-101.
- Meagher RL, Epsky ND, Cherry R. 2007. Mating behavior and female-produced pheromones use in tropical sod webworm (Lepidoptera: Crambidae). Fla Entomol. 90: 304−308. https://doi.org/10.1653/0015-4040(2007)90[304:MBAFPU]2.0.CO;2.
- Niemczyk HD. 1981. Destructive turf insects. HDN Book Sales. Wooster, OH. 48 pp.
- Noosidum A, Hodson AK, Lewis EE, Chandrapatya A. 2010. Characterization of new entomopathogenic nematodes from Thailand: foraging behavior and virulence to the Greater wax moth, Galleria mellonella L. (Lepidoptera: Pyralidae). J Nematol. 42: 281−291.
- Racke KD. 2000. Pesticide for turfgrass pest management uses and environmental issues. Pp-45. In: Clark M and M. P. Kennum MP (Eds.), Fate and Management of turf grass chemicals. https://doi.org/10.1021/bk-2000-0743.ch003.
- Reinert JA, Engelke MC, Genovesi AD, Chandra A, McCoy JE. 2009. Resistance to tropicalsod webworm (Herpetogramma phaeopteralis) (Lepidoptera:Crambidae) in St. Augustine grass and zoysia grass. Intl Turfgrass Society Res J. 11: 663−673.
- Shapiro-Ilan DI, Blackburn D, Duncan L, El-Borai FE, Koppenho¨fer H, Tailliez P, Adams BJ. 2014. Characterization of biocontrol traits in Heterorhabditis floridensis: A species with broad temperature tolerance. J Nematol. 46(4): 336−345.
- Tofangsazi N, Cherry RH, Arthurs SP. 2014. Efficacy of commercial formulations of entomopathogenic nematodes against tropical sod webworm, Herpetogramma phaeopteralis (Lepidoptera: Crambidae). J Appl Entomol. DOI: 10.1111/jen.12125. https://doi.org/10.1111/jen.12125.
- White GF. 1927. A method for obtaining infective nematode from cultures. Science 66: 302− 303. https://doi.org/10.1126/science.66.1709.302-a.
- Woodring LJ and Kaya KH. 1988. Steinernematid and Heterorhabditid nematodes. A handbook of biology and techniques. Southern Cooperative Series Bulletin. A publication ofthe nematode subcommittee of the Southern Research Project S135- Entomopathogens foruse in Pest Management Systems.